Experiment
Composite interval mapping (CIM) was performed to identify QTL's involved in experimental allergic encephalomyelitis (EAE), the principal animal model for multiple sclerosis (MS). 173 microsatellite markers were typed in 673 animals from a (B10.S/DvTe x SJL/J)F2 intercross. The SJL/J strain is susceptible to EAE whereas B10S.DvTe is resistant. The authors report 9 novel QTLs in this study, some of which appear to be sex-influenced. Eae15, which affects brain lesion severity, maps to Chromosome 10 from 4 - 19 cM with a LRT (Likelihood Ratio Test Statistic) score of 26.26 in linkage with D10Mit2. Eae16, influencing brain lesion severity (LRT = 19.74) and mononuclear infiltration (LRT = 27.27), maps to Chromosome 12 from 3 - 29 cM in linkage with D12Mit12. Eae20, influencing demyelination (LRT = 17.21), maps to Chromosome 3 from 4 - 23 cM in linkage with D3Mit55. Eae21 and Eae22, QTLs influencing lesion severity (LRT = 25.71 for Eae21; 20.21 for Eae22) and mononuclear infiltration (LRT = 25.90 for Eae21; 16.64 for Eae22) in female mice, mapped to Chromosome 2 from 30 cM - 69 cM in linkage with D2Mit9, and Chromosome 11 from 49 cM - 71 cM in linkage with D11Mit330, respectively. Eae23, influencing lesion severity (LRT = 21.05) and mononuclear infiltration (LRT = 18.95) in male mice, maps to Chromosome 11 from 32 cM - 44 cM in linkage with D11Mit155 and D11Mit194. Eae16 and Eae19, QTLs influencing demyelination (LRT = 17.92 for Eae16; 19.31 for Eae19) in male mice, mapped to Chromosome 12 from 3cM - 29cM in linkage with D12Mit12, and chromosome 19 from 26 cM - 50 cM in linkage with D19Mit05, respectively. The SJL/J allele increases severity of phenotypes (brain lesion, mononuclear infiltration, and demyelination) at all QTLs discussed in this study.
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