Experiment
The authors analysed 38 animals from a backcross and 28 animals from an intercross of In55Rk stock mice with C57BL/6J mice. D3Mit324 and D3Mit92 cosegregate with the inversion and estimate the proximal breakpoint region. D3Mit127, D3Mit160 and D3Mit200 also cosegregate with the inversion and estimate the distal breakpoint region. The interlocus distance based on the genetic analyis was determined to be: D3Mit160 - 2.1 +/- 1.5 - D3Mit164 - 7.5 +/- 2.7 - D3Mit324, D3Mit92, In(3)55Rk-p - 2.1 +/- 1.5 - D3Mit118 - 1.1 +/- 1.1 - D3Mit21 - 4.3 +/- 2.1 - D3Mit69 - 5.3 +/- 2.3 - D3Mit29 - 9.6 +/- 3.0 - D3Mit350 - 1.1 +/- 1.1 - D3Mit351 - 2.1 +/- 1.5 - D3Mit127, D3Mit160, D3Mit200, In(3)55Rk-d - 2.1 +/- 1.5 - D3Mit147 - 19.2 +/- 4.1 - D3Mit129. The mapping order here within the inverted segment is represented as normal. However, cytologically the marker order has been shown to be inverted between D3Mit324 and D3Mit200. The distance encompassed by the inversion in this genetic cross was determined to be 25.5 cM. However, comparison of this distance to the MGI genetic map indicated that the distance between the markers was 72.8 cM. This highlights the suppression of recombination caused by the inversion.
Analysis Tools