Mapping Data

Experiment

• Experiment
  TEXT-Genetic Cross
• Chromosome
  4
• Reference
  J:68682 Li X, et al., High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal Chromosome 4. Mamm Genome. 2001 Jan;12(1):13-6
• ID
  MGI:1933950

Genes

Gene	Assay Type
D4Mit33	PCR
D4Mit190	PCR
D4Mit42	PCR
Tas1r1	PCR
D4Mit254	PCR
D4Mit209	PCR
D4Mit256	PCR
Pusl1	PCR
Tas1r3	visible phenotype

Notes

• Experiment
  A Total number of 1258 (629 F2 mice) chromosomes were typed using a DNA mapping panel from an F2 intercross between 129P3/J and C57BL/6ByJ strains. The animals were produced from reciprocal outcrossing of 129P3/J females and C57BL/6ByJ males, or C57BL/6ByJ females and 129P3/J males. The following order of loci on mouse chromosome 4 was indicated: Centromere - D4Mit33 - 2.5 cM - D4Mit190 - 1.5 cM - D4Mit42 - 0.2 cM - Gpr70 - 1.1 cM - D4Mit254 - 0.8 cM - D4Mit209 - 3.2 cM - D4Mit256 - 2.6 cM - D18346 - Telomere. Authors use these data along with marker analysis in congenic 129.B6-Sac to show that the mouse Chromosome 4 locus Sac is not encoded by Gpr70. Moreover, mouse gene Tas1r1 is coded at Gpr70 locus.
  
  The authors in a submission to MGI indicated the following:
  
  NOTE discrepancies between the MGD map and panel data:
  a) Our data resolve positions of D4Mit33 and D4Mit190, both mapped to 79.0 cM by MGD.
  b) Our data place D4Mit209 more distal (between D4Mit254 and D4Mit256) compared with the MGD map (proximal to D4Mit42). Our data for D4Mit209 are also consistent with the MIT map.