Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  10
• Reference
  J:54925 Gershenfeld HK, et al., Mapping quantitative trait loci for seizure response to a GABAA receptor inverse agonist in mice. J Neurosci. 1999 May 15;19(10):3731-8
• ID
  MGI:1343949

Genes

Gene	Assay Type
D10Mit180
D10Mit237
Exq1	visible phenotype

Notes

• Experiment
  The authors examined the gentic basis of differences in seizure behaviour using a GABAa receptor agoinst beta CCM (methyl beta-carboline-3 carboxylate). A/J mice are more susceptible to beta-CCM induced myoclonic seizures than C57BL/6J mice in both latency to seizure and proportion of tested mice that seized. A genome wide screen in an F2 population of these parental strains identified a locus on distal Chromosome 10, near D10Mit180, that displayed an association with seizure susceptibility (chi square =15.77; p = 0.004; n = 271) and seizure latency. A LOD score = 4.29 was assigned to this locus and it accounted for ~25% of the genetic variance and 5.7% of the phenotypic variance for this seizure trait. The best-fitting logistic regression model suggeststhat the A/J allele at the D10Mit180 locus increases the likelihood of seizures at ~2.7 fold. The Chromosome 10 QTL was confirmed in a subsequent backcross population (n = 223). D10Mit237 which is located ~4 cM distal to D10Mit180 displayed significant association to seizure susceptibility (chi square = 19.30; p = <0.0001; n = 214) and seizure latency. A LOD score = 4.36 was assigned to this locus an it accounted for 6-9% of the phenotypic variance for this seizure trait. The best-fitting logistic regression model suggests that the C57BL/6J allele at the D10Mit237 locus increased the likelihood of resistance to seizures ~2.8 fold. This Chromosome 10 locus has been named Exq1 because it appears to affect both O-F exploratory behaviour and seizure susceptibility. No known candidate genes are in the telomeric 15 cM region of mouse Chromosome 10.