Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  13
• Reference
  J:54407 Hong HK, et al., Pleiotropic skeletal and ocular phenotypes of the mouse mutation congenital hydrocephalus (ch/Mf1) arise from a winged helix/forkhead transcriptionfactor gene. Hum Mol Genet. 1999 Apr;8(4):625-37
• ID
  MGI:1341568

Genes

Gene	Assay Type	Description
D13Mit206	overlapping clones
D13Cwr32	overlapping clones
D13Cwr31	overlapping clones
Foxq1	overlapping clones, PCR amplified length variant	Hfh1-pA, Hfh1-pB, Hfh1-pC, hfh1-pD
D13Cwr30	overlapping clones
D13Cwr27	overlapping clones
D13Cwr26	overlapping clones
D13Cwr25	overlapping clones
D13Cwr24	overlapping clones
Foxc1	overlapping clones
D13Cwr23	overlapping clones
D13Cwr22	overlapping clones
D13Cwr21	overlapping clones
D13Mit294	overlapping clones
D13Cwr20	overlapping clones
D13Cwr19	overlapping clones
D13Cwr18	overlapping clones
D13Cwr17	overlapping clones
D13Cwr16	overlapping clones
D13Cwr15	overlapping clones
D13Cwr14	overlapping clones
D13Cwr13	overlapping clones
D13Cwr12	overlapping clones
D13Cwr11	overlapping clones
D13Cwr10	overlapping clones
D13Cwr9	overlapping clones
D13Cwr8	overlapping clones
D13Cwr7	overlapping clones
D13Cwr6	overlapping clones
D13Cwr5	overlapping clones
D13Cwr4	overlapping clones
D13Mit307	overlapping clones
D13Cwr3	overlapping clones
D13Cwr2	overlapping clones
D13Cwr1	overlapping clones

Notes

• Experiment
  The authors constructed an YAC/BAC contig around the ch locus on mouse Chromosome 13. Several BAC and YAC end clones were cloned and used as STSs to aid in the construction of the physical map. They identified the Mf1 gene in this region. Sequencing of the Mf1 gene revealed a nonsense mutation responsible for the ch phenotype. The authors also used Hfh1 specific primers to detect the Hfh1 gene in the region. Hfh1 was placed proximal to Mf1. The order of markers on mouse Chromosome 13 is: D13Mit206 - D13Cwr32 - D13Cwr31 - Hfh1 - D13Cwr30 - D13Cwr27 - D13Cwr26 - D13Cwr25 - D13Cwr24 - Mf1 - D13Cwr23 - D13Cwr22 - D13Cwr21 - D13Mit294 - D13Cwr20 - D13Cwr19 - D13Cwr18 - D13Cwr17 - D13Cwr16 - D13Cwr15 - D13Cwr14 - D13Cwr13 - D13Cwr12 - D13Cwr11 - D13Cwr10 - D13Cwr9 - D13Cwr8 - D13Cwr7 - D13Cwr6 - D13Cwr5 - D13Cwr4 - D13Mit307 - D13Cwr3 - D13Cwr2 - D13Mit265 - D13Cwr1.