Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  4
• Reference
  J:50273 Poltorak A, et al., Genetic and physical mapping of the Lps locus: identification of the toll-4 receptor as a candidate gene in the critical region. Blood Cells Mol Dis. 1998 Sep;24(3):340-55
• ID
  MGI:1340510

Genes

Gene	Allele	Assay Type
L22T7		overlapping clones
D4Mit325		overlapping clones
L22SP6		overlapping clones
O12T7		overlapping clones
CA25.5		overlapping clones
D4Mit25		overlapping clones
CA25.15		overlapping clones
CA25.2		overlapping clones
C.seq		overlapping clones
O12SP6		overlapping clones
O20SP6		overlapping clones
CA9.1		overlapping clones
CA9.2		overlapping clones
N13T7		overlapping clones
NDS9		overlapping clones
M6T7		overlapping clones
O20T7		overlapping clones
B.seq		overlapping clones
K20T7		overlapping clones
M6SP6		overlapping clones
K20SP6		overlapping clones
B6T7		overlapping clones
C8SP6		overlapping clones
D4Mit178		overlapping clones
F.seq		overlapping clones
B6SP6		overlapping clones
A.seq		overlapping clones
D.seq		overlapping clones
E.seq		overlapping clones
I16T7		overlapping clones
I16SP6		overlapping clones
F15SP6		overlapping clones
G.seq		overlapping clones
O21SP6		overlapping clones
N19T7		overlapping clones
CA7.15		overlapping clones
CA178.8		overlapping clones
CA7.1		overlapping clones
CA7.3		overlapping clones
CA7.2		overlapping clones
J14SP6		overlapping clones
CA7.4		overlapping clones
CA7.7		overlapping clones
CA7.6		overlapping clones
D4Mit7		overlapping clones
B5T7		overlapping clones
N15SP6		overlapping clones
B22SP6		overlapping clones
CA7.13		overlapping clones
CA7.11		overlapping clones
CA7.5		overlapping clones
CA7.12		overlapping clones
CA7.10		overlapping clones
O1T7		overlapping clones
CA7.14		overlapping clones
CA7.17		overlapping clones
CA7.18		overlapping clones
H9SP6		overlapping clones
D24SP6		overlapping clones
CAH9.3		overlapping clones
K4SP6		overlapping clones
D4Mit132		overlapping clones
K4T7		overlapping clones
D4Mit83		overlapping clones
CA83.3		overlapping clones
Tlr4	Tlr4^Lps-d	visible phenotype
Pappa		overlapping clones

Notes

• Experiment
  A series of BAC ad YAC clones were aligned to create a physical map of the Lps^d (endotoxin-unresponsive) phenotypic region. Comparing the physical map with backcross data indicates a 2.6 Mb region bound by markers B and CA83.3 representing the maximum limits of locus confinement. Within this region, a 1.2 Mb "zero recombination region" is defined by backcross data. Tlr4 the only complete gene identified by sequencing with in the 2.6 Mb region, was anticipated to be the responsible gene. Such was provento be the case in later publications. The order of regional loci reported here is: L22T7 - D4Mit325 - L22SP6 - O12T7 - CA25.5 - D4Mit25 - B6T7 - C8SP6 - D4Mit178 - F - B6SP6 - (A, D, E) - I16T7 - Il6SP6 - F15SP6 - G - O21SP6 - N19T7 - CA7.15 - CA178.8 - CA7.1 - CA7.3 - CA7.2 - J14SP6 - CA7.4 - CA7.7 - CA7.6 - D4Mit7 - B5T7 - N15SP6 - B22SP6 - CA7.13 - CA7.11 - (CA7.5, CA7.12, CA7.10) - O1T7 - (CA7.14, CCA7.17, CA7.18) - H9SP6 - D24SP6 - CAH9.3 - K4SP6 - D4Mit132 - KAT7 - D4Mit183 - CA83.3. The 5' portionof Pappa was found in the proximal portion of the 2.6 Mb region, transcribed in the opposite direction of Tlr4.