Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  6
• Reference
  J:55124 Idris AH, et al., The natural killer gene complex genetic locus Chok encodes Ly-49D, a target recognition receptor that activates natural killing. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6330-5
• ID
  MGI:1340210

Genes

Gene	Allele	Assay Type
Klra4	Klra4^Chok	visible phenotype

Notes

• Experiment
  The authors had previously localized the Chok (CHO cell killing) phenotype to the natural killer (NK) gene complex on mouse Chromosome 6. using a lectin-facilitated lysis assay, the authors identified a monoclonal antibody (mAb 4E4) that blocked B6 - mediated CHO lysis. Flo cytometry indicated that mAb 4E4 reacted specifically with Klra4. Subsequent experiments involving transfer of the Klra4 gene into BALB/c NK cells conferred the Chok phenotype to these cells. The authors were thus able to show that the Chok phenotype was due to the C57BL/6 Klra4 gene on mouse Chromosome 6.