Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  2
• Reference
  J:48583 Zuberi AR, et al., Expression screening of a yeast artificial chromosome contig refines the location of the mouse H3a minor histocompatibility antigen gene. J Immunol. 1998 Jul 15;161(2):821-8
• ID
  MGI:1309633

Genes

Gene	Assay Type
D2Dcr20	overlapping clones
D2Mit444	overlapping clones
D2Dcr17	overlapping clones
D2Dcr15	overlapping clones
Rad51	overlapping clones
D2Mit133	overlapping clones
D2Dcr14	overlapping clones
D2Dcr19	overlapping clones
D2Dcr16	overlapping clones
D2Dcr22	overlapping clones
D2Mit395	overlapping clones
Ltk	overlapping clones
D2Dcr23	overlapping clones
Tyro3	overlapping clones
Capn3	overlapping clones
D2Dcr21	overlapping clones
Epb42	overlapping clones
D2Dcr24	overlapping clones
D2Mit190	overlapping clones
D2Dcr11	overlapping clones
D2Dcr6	overlapping clones
D2Dcr7	overlapping clones
D2Dcr8	overlapping clones
D2Dcr13	overlapping clones
D2Dcr18	overlapping clones
Ckmt1	overlapping clones
D2Dcr12	overlapping clones
D2Dcr2	overlapping clones
D2Dcr5	overlapping clones
B2m	overlapping clones
D2Dcr4	overlapping clones
n-THgtg5	overlapping clones
D2Dcr10	overlapping clones
D2Dcr3	overlapping clones
D2Dcr9	overlapping clones
D2Mit17	overlapping clones
Zfp106	overlapping clones

Notes

• Experiment
  Overlapping clones were aligned to physically map the H3a region of mouse Chromosome 2. Chimerism and Chromosome 2 localization of YAC ends was determined by PCR amplification of genomic DNA and hamster-mouse somatic cell monochromosomal hybrids that contain only mouse Chromosome 2. Additional confirmation was derived using the JAX BSS [(C57BL/6JEi x SPRET/Ei)F1 x SPRET/Ei] panel or a panel of (B10 x 129)F1 variants that demonstrate loss heterozgosity around the Zfp106 locus. The following order of loci was reported: D2Dcr20 - D2Mit444 - D2Dcr17 - D2Dcr15 - Rad51 - D2Mit133 - D2Dcr14 - D2Dcr19 - D2Dcr16 - D2Dcr22 - D2Mit395 - Ltk - D2Dcr23 - Tyro3 - Capn3 - D2Dcr21 - Epb4.2 - D2Dcr24 - D2Mit190 - D2Dcr11 - D2Dcr6 - D2Dcr7 - D2Dcr8 - D2Dcr13 - D2Dcr18 - Ckmt1- D2Dcr12 - D2Dcr2 - D2Dcr5 - B2m - D2Dcr4 - Trnah - D2Dcr10 - D2Dcr3 - D2Dcr9 - D2Mit17. Analysis of YAC expression indicates that Sh3bp3 most likely maps proximal of Epb4.2. Loss of heterozygosity on clone M3H6 supports the mapping of Zfp106 in close proximity to Capn3. Tyro3 and Capn3 are reported to be oriented similarly on the chromosome with transcription proceeding from the centromere to the telomere.