Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  2
• Reference
  J:47466 Chambers DM, et al., The lethal mutation of the mouse wasted (wst) is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 gene. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4463-8
• ID
  MGI:1276433

Genes

Gene	Allele	Assay Type	Description
Eef1a2	Eef1a2^wst	direct sequencing	1r, 3f, Eef1a2 genomic

Notes

• Experiment
  The authors designed primers to amplify each exon of the mouse Eef1a2 gene in wst/wst mice and wild type HRS/J mice. While exons 3-8 were amplified in both sets of mice, exons 1 and 2 were not detected in wst/wst mice. The authors then used subcloning, direct sequencing and further PCR amplification to define the wasted deletion to a 15.8kb region. One end of the deletion lies 206 bp upstream of exon 2 of the Eef1a2 gene and the other end is in the gag gene of the IAP element.