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| Caption | Abnormal lymphatic development in Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ mice. (A) X-gal staining (blue) of E13.5 Flt4tm1Ali/Flt4+ (vegfr3+/-, left) and double-heterozygous Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-, right) littermate embryos. (B) Expression levels of lymphatic marker genes as measured by quantitative PCR in RNA isolated from hearts of wild-type (WT) and double-heterozygote Nrp2tm1Ddg/Nrp2+ Kdrtm1.1Eia/Kdr+ (nrp2+/- vegfr2+/-) and Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-) mice. Values significantly different from WT mice (*, P > 0.05; **, P > 0.001) by Student's t test are shown. Error bars indicate SEM. (C and D) Higher magnification of heads of embryos shown in A. Note numerous lymphatic vessel sprouts in Flt4tm1Ali/Flt4+ (vegfr3+/-) (black arrows) and fewer enlarged lymph vessel sprouts in Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-) (red arrows). e, eye; *, ear. (E and F) Transverse section through the neck of E13.5 embryos double stained with X-gal (blue) and CD-31 (brown). Note similar development of CD-31-positive arteries, veins, and skin capillaries (arrowheads) in Flt4tm1Ali/Flt4+ (vegfr3+/-) (E) and Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-) (F). Note enlarged jugular lymph sacs (asterisks) in Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-) (F) compared with Flt4tm1Ali/Flt4+ (vegfr3+/-) (E). Lymph vessels sprouting from the lymph sac toward the skin are less numerous in Nrp2tm1Ddg/Nrp2+ Flt4tm1Ali/Flt4+ (nrp2+/- vegfr3+/-) compared with Flt4tm1Ali/Flt4+ (vegfr3+/-) (arrows). A, arteries; V, veins; NT, neural tube; Vt, vertebra. Bars: (A) 1.4 mm; (C and D) 0.8 mm; (E and F) 150 um. | ||||||
| Copyright | This image is from Xu Y, J Cell Biol 2010 Jan 11;188(1):115-30, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:156869 | ||||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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