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| Caption | A & B: Immunodetection of BrdUrd-labeled cells (brown dots) with lower amount of proliferative cells in the proliferative zone (arrows) of 2-wk-old Mmp14tm1Ktry/Mmp14tm1Ktry mice compared with those of wild type littermates. C & D: Comparative histology of growth plates of the distal femur in 15 day old mutant and wild type mice. Note the disorganized structure and low cell number in the proliferative zone in mutant mice. The lack of vascularization and bone formation in the epiphyseal secondary ossification center of mutant mice can also be observed. E & F: Comparison of secondary ossification center of the distal femur from 15-day-old mice. There is advanced ossification in wild type mice (F), whereas hypertrophic chondrocytes persist in the location of the ossific center (*) of the mutant mice, with no signs of ossification (E). C-F: Modified Movat pentachrome stain; PZ,= proliferative zone; HZ, hypertrophic zone. G & H: Immunohistochemical staining with antibodies against PECAM revealing lack of endothelial cells in the epiphysis of mutant mice (G), whereas positive staining is seen throughout the epiphysis in wild type mice, as shown to be located in endothelial cells of blood vessels (G, Inset). Note the similar PECAM staining at the chondroosseous junction in mutant and wild type mice. Arrows indicate areas magnified in insets. Arrowheads in the insets depict blood vessels (H). VEGF (I and J) and its receptor Flk-1 (K and L) reveal no evidence for vascularization in the secondary ossification zone of the femoral epiphysis in mutant mice. Insets in G-L show higher magnifications taken from locations shown by arrows. Arrowheads in insets of I-L indicate VEGF or Flk-1-positive cells, and arrows in the insets depict the positively stained blood vessels. | ||||
| Copyright | This image is from Zhou Z, Proc Natl Acad Sci U S A 2000 Apr 11;97(8):4052-7. Copyright 2000 National Academy of Sciences, U.S.A. J:61685 | ||||
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Associated Alleles |
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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