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| Caption | Generation of the Trim3tm1Mak allele. (A) Schematic representations of the wild-type Trim3 allele (Top), the targeting construct showing the replacement of most of coding exons 2-5 with a neomycin resistance cassette and the introduction of an additional NcoI site (Middle), and the mutant allele (Trim3tm1Mak) after successful homologous recombination (Bottom). The 5'-flanking probe (fp) located external to the targeting construct was used to identify successfully recombined ES cell clones as well as to confirm mouse genotypes by Southern hybridization. Arrowheads a/c and b/c indicate the primers used for PCR in B. | ||||
| Copyright | This image is from Cheung CC, Proc Natl Acad Sci U S A 2010 Jun 29;107(26):11883-8. Copyright 2010 National Academy of Sciences, U.S.A. J:161372 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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