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| Caption | Generation of the Ghsrtm1Mws allele. Bacterial artificial chromosome for Ghsr was isolated, and targeting vectors in which exon 1 of the Ghsr locus was precisely deleted and replaced with an in-frame lacZ reporter gene and neomycin-selectable marker were electroporated into embryonic stem cells. Correctly targeted embryonic stem cells, as well as eventual heterozygotes and homozygous mice derived from these embryonic stem cells, were identified by a real-time PCR-based loss-of-native-allele assay. After germ-line transmission was established, F1 heterozygous mice were backcrossed to C57BL6/J to generate N3 breeding heterozygote pairs that were used to generate homozygous wild-type (WT) and mutant mice used in these experiments. Deletion of Ghsr was also confirmed by the absence of exogenous ghrelin-induced feeding response. | ||||
| Copyright | This image is from LeSauter J, Proc Natl Acad Sci U S A 2009 Aug 11;106(32):13582-7. Copyright 2009 National Academy of Sciences, U.S.A. J:152003 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/21/2024 MGI 6.23 |
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