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| Caption | Generation of the Gjc2tm2.1Kwi (Cx47M282T) allele. A, Scheme of homologous recombination of the targeting vector into the Gjc2 (Cx47) coding region. The resulting transgenic allele (Cx47M282Tneo) includes mCx47M282T coding DNA, an internal ribosome entry site (IRES) followed by a nuclear localization signal (nls) fused to LacZ coding DNA and a neomycin selection cassette flanked by frt-sites. The endonuclease BsmBI restriction site was generated by T to C transition on nucleotide 845 of the Gjc2 coding region, resulting in the mutant mCx47M282T. B, Flp recombinase activity causes deletion of the neomycin selection cassette resulting in the Gjc2tm2.1Kwi allele. Specific primer binding sites and MfeI restriction sites are indicated. C, PCR products specific for wild-type (570 bp) and transgenic loci (730 bp) using DNA obtained from tail tip tissue. | ||||
| Copyright | This image is from Tress O, PLoS Genet 2011 Jul;7(7):e1002146, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174197 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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