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| Caption | Generation of the Tgfb1tm1Mki and Tgfb1tm1.1Mki alleles. (A) The targeting strategy. (A, line A) The target locus is Tgfb1. Coding sequences and the endogenous 3'-UTR of the Tgfb1 gene are shown as black and white columns. (A, line B) The targeting vector contains a loxP sequence, the c-Fos 3'-UTR (blue), two copies of a transcriptional insulator (cHS) from the chicken Beta-globin gene (yellow), a Neo gene with the MC1 promoter (pMC1), loxP, and the 3'-UTR of the bovine growth hormone gene (bGH3'-UTR, red). The insulators minimize interference between the Tgfb1 promoter and pMC1. TK, thymidine kinase gene. (A, line C) The locus after homologous recombination. TGFBeta1 expression is now controlled by the 3'-UTR of Fos [low (L) allele, Tgfb1tm1Mki], which destabilizes the TGFBeta1 mRNA and decreases TGFBeta1 protein. (A, line D) The locus after Cre-lox P recombination. TGFBeta1 expression is controlled by the 3'-UTR of bGH [high (H) allele], Tgfb1tm1.1Mki, which stabilizes TGFBeta1 mRNA and increases TGFBeta1 protein. Gene targeting was confirmed by PCR with primers (F and R) and by a Southern blot with tail genomic DNA digested by Nco I radiolabeled with a probe (P). | ||||||
| Copyright | This image is from Kakoki M, Proc Natl Acad Sci U S A 2013 Apr 2;110(14):5600-5. Copyright 2013 National Academy of Sciences, U.S.A. J:194237 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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