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| Caption | Generation of the Snord50a/Snord50btm1.1Soen allele. Schematic representation of the targeting strategy based on the wild-type allele. In the targeting vector, the first mU50 snoRNA sequence (upstream) is substituted by the 68 bp multiple cloning site of a pBluescriptII KSII plasmid containing an EcoRV recognition site (EV). The second mU50 snoRNA sequence (downstream) is replaced with a loxP-Neor-loxP cassette. The pair of facing arrows indicates the set of forward/reverse primers used for genotyping PCR. DT: diphtheria toxin negative selection cassette; Neor: neomycinresistant gene cassette; Cre: treatment with Cre recombinase; SB probe: a probe for Southern blot. | ||||||
| Copyright | This image is from Soeno Y, PLoS One 2013;8(8):e72105, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:206419 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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