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| Caption | Generation of the Ptk2tm1Heen allele. (A) Structure and restriction maps of the wild-type mouse gene, targeting vector and the obtained allele. Constitutive and alternative exons are figured by dark or white vertical boxes respectively, with their number indicated below. The insertion of a pgk-hygromycin (hygro)-resistance cassette flanked with FRT sites (ovals) allowed the deletion of alternative exons 13 and 14, and the positive selection of the homologous recombination event. Note that deletion of exons 13 and 14 is expected to have no consequence during early embryogenesis since they are not detected in mRNA during the first part of embryonic development. The LoxP sequences (triangles) flanking the alternative exon 16 were placed for possible conditional deletion of this exon by the Cre recombinase. The position of the 5' and 3' probes used for Southern blots are indicated. Arrows represent the position of the primers (P1, P2, P3) used for genotyping by PCR. Abbreviations for the restriction sites are: P: PstI, M: MstII, B: BamH1, A: BstI, X: XmnI, G: BglII, S: SfiI. | ||||
| Copyright | This image is from Corsi JM, J Biol Chem 2009 Dec 11;284(50):34769-76 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:157804 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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