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| Caption | Generation of the Ccr5tm2Blck allele. (b) Schematic representation of the targeting strategy used for the inactivation of the Ccr5 gene. The Ccr5 wild-type locus, the targeting vector and the targeted locus before and after Cre-mediated recombination are shown together with restriction sites relevant for Southern blot analysis. White boxes represent 5'- and 3'-untranslated regions, the blue box the coding region of the Ccr5 gene. Thick lines indicate homology arms, and the loxP-flanked hygromycin (hyg) selection cassette is depicted as a yellow box with black triangles at the ends symbolizing the loxP sites. The wavy line represents prokaryotic vector sequences in the targeting vector. The DsRed2 (red fluorescent protein) reporter gene at the targeted locus is shown as a red box. Sizes of the relevant restriction fragments or PCR products before and after homologous recombination and Cre-mediated deletion are shown. Arrowheads indicate the positions of primers used to demonstrate the deletion of the hygromycin cassette by long-range PCR. | ||||
| Copyright | This image is from Luckow B, Genesis 2009 Aug;47(8):545-58, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:152815 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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