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| Caption | Generation of the Col10a1tm1.1Rpbh allele. (A) Targeting strategy: (i) Wild-type collagen X allele indicating location of probes used for Southern blotting and relevant restriction enzyme sites; exons are numbered boxes and the region of exon 3 encoding the collagenous domain is indicated by dappling; (ii) Targeting construct with p.Asn617Lys (N617K) mutation (*) and floxed Neo TK selection cassette; (iii) Recombinant allele identified using the external probe; (iv) Mutant knock-in allele containing the p.Asn617Lys mutation and residual LoxP site following deletion of the Neo TK cassette by transient Cre transfection and FIAU selection. | ||||
| Copyright | This image is from Rajpar MH, PLoS Genet 2009 Oct;5(10):e1000691, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:154052 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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