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| Caption | Generation of the Cryba1tm1a(EUCOMM)Hmgu allele. Schematic of the first knockout strategy used for generating knock-out mice. Shown is the Cryba1tm1a(EUCOMM)Hmgu knockout first allele with a (FRT)-flanked selection cassette inserted into the third intron of the Cryba1 (BetaA3/A1) gene, which traps the transcript through the Engrailed-2-splice acceptor (En2 sA) and truncates it through the SV40 polyadenylation signal. Primer specific to the neomycin resistance gene was used to confirm the disruption of the gene. | ||||||
| Copyright | This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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