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| Caption | Generation of the Igs2tm3.1Luo and Igs2tm4Luo alleles. (i) A schematic of the recombinant DNA construct for introduction of three attP sites and the NVPhi cassette into H11 via homologous recombination in mouse ES cells. Two versions, containing either a single "full-length" attP site or three tandem short attP sites, were generated. (ii) mouse chromosome 11 with the Hipp11 (H11) locus designated as a red triangle. The scale below is in kilobases, except where megabases (Mb) are indicated. (iii) The H11P3NVPhi allele resulting from homologous recombination between (i) and (ii). (iv) H11P3-pCA-GFP-BB-NVPhi allele obtained by PhiC31-catalyzed site-specific insertion of the pattB-pCA-GFP plasmid (pBT316) into the first attP site. All three attP sites are suitable recipients for the transgene and the site used in any particular case can be determined by PCR. (v) H11P3 locus that was generated from (iii) by Flpo mRNA injection into the cytoplasm of mouse embryos carrying (iii). The H11 locus with a single attP site and the Rosa26 locus with either a single or three attP sites were generated in the same manner. (vi) and (vii), two products obtained by PhiC31-catalyzed site-specific insertion of the pattB-pCA-GFP plasmid (pBT316, left) or the attB-pCA-GFP minicircle (generated from pBT346, right). The corresponding alleles are: H11P3-pCA-GFP-BB (left) and H11P3-pCA-GFP (right), respectively. | ||||||
| Copyright | This image is from Tasic B, Proc Natl Acad Sci U S A 2011 May 10;108(19):7902-7. Copyright 2011 National Academy of Sciences, U.S.A. J:171979 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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