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| Caption | Generation of the Snap23tm1Paro (Snap23 Neo+fl), Snap23tm1.1Paro (Snap23delta), and Snap23tm1.2Paro (Snap23fl) alleles. (A)Schematic representation of the genomic structure of wild-type and targeted alleles of Snap23. Homologous recombination with the targeting construct inserts a neomycin-resistance gene (PKG-NEO) and exon 2 (E2) flanked by three loxP sites represented as triangles. HSK-tk was used for negative selection. Exon numbers with relative position, 5'- and 3'- flanking Southern blotting, and a partial restriction map are indicated. Restriction sites: C, ClaI; E, EcoRI; K, KpnI; S, SpeI. Female Snap23Neo+fl/wt heterozygous mouse (Snap23tm1Paro) harboring the targeted allele (F1) was bred with male EIIa-Cre transgenic mice. Offspring harboring mosaic alleles (F2) through partial and/or total excision of loxP-flanked sequences were generated. EIIa-Cre+ F2 mosaic male mice were further bred with C57BL/6 female mice to separate the Snap23fl/wt, Snap23Neo+fl/wt, and Snap23Delta/wt alleles as shown (F3) | ||||||||
| Copyright | This image is from Suh YH, PLoS One 2011;6(3):e18444, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171444 | ||||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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