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| Caption | Generation of the Del(8Defb1-Defb13)1Jrdo deletion. MICER vectors target two regions of the chromosome 8 beta-defensin cluster in embryonic stem (ES) cells. Gene orientation indicated with solid arrows. First targeting vector carrying neomycin resistance (neo) and Hypoxanthine-guanine phosphoribosyltransferase (HPRT) exons 1 and 2 and tyrosinase (tyr), second targeting vector carrying puromycin resistance gene (puro), Agouti (Ag) and exons 3-9 of HPRT. DNA homologous to the genomic region indicated by black within the vectors. Following cre recombinase treatment of the double targeted cells recombination between the lox sites (solid triangles) results in a functional HPRT gene and E14 cells (normally Hprt mutant) that can now grow on hypoxanthine aminopterin thymidine (HAT) medium but are both puromycin and G418 sensitive. Vectors not drawn to scale. | ||||
| Copyright | This image is from Zhou YS, PLoS Genet 2013 Oct;9(10):e1003826, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:204187 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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