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| Caption | Generation of the Rxfp4tm1Sora allele. To generate the targeting construct 3,125-bp 5' and 1,810-bp 3' homology arms were PCR amplified from genomic DNA and cloned into a vector containing an IRES-beta-gal reporter gene, a Neomycin selection marker under the control of a MC1 promoter, and two Thymidine Kinase negative selection markers. The linearized vector was electroporated into ES cells. Homologous recombination was detected by screening PCR and SspI-digested Southern blotting of genomic DNA of individual clones as well as the parental cell line wild-type (wt). Fig. | ||||
| Copyright | This image is from Grosse J, Proc Natl Acad Sci U S A 2014 Jul 29;111(30):11133-8. Copyright 2014 National Academy of Sciences, U.S.A. J:212239 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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