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| Caption | The "RasE Multi-Hit BAC" transgene construct. Overall structure (top) and individual expression modules (bottom). Each expression module is in the inverted (off) position relative to its CAG promoter (P); the expressed sequence is flanked by loxP sites (triangles), oriented head-to-head, so it can be activated by Cre recombinase-mediated "flipping." Deletion of sequences between similarly-oriented loxP sites is prevented by use of incompatible, heterotypic loxP sites for different modules: black represents canonical loxP, blue lox5171, and red lox2272. Other components: FRT, FRT recombinase recognition site; 2xHS4, chicken 2xHS4 insulator sequence; pA, SV40 polyadenylation signal; Gtx, internal ribosomal entry site; eYFP, dsRed, and hCD2t, fluorescent protein reporter genes; S35, G37, and C40, the MAPK, RALGEF and PI3K pathway-activating HrasG12V mutant alleles. | ||||
| Copyright | This image is from Musteanu M, Nat Methods 2012 Sep;9(9):897-900, and is displayed with the permission of The Nature Publishing Group who owns the Copyright. J:226601 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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