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| Caption | Generation of the Ilktm10.1Ref allele. (A) Scheme of the murine Ilk locus with black rectangles indicating exons 12 and 13, knock-in mutations in exon 12 are indicated. The indicated point mutations were generated in a PAC (P1 artificial chromosome)-derived fragment of the ILK locus by site-directed mutagenesis. A neomycin selection cassette (neo) flanked by loxP sites (triangles) was introduced into the 3' untranslated region of exon 13 to select for positive homologous recombinant R1 embryonic stem (ES) cell clones. Correct homologous recombination was tested by Southern blotting of EcoRV-digested genomic DNA with an external probe, which binds to a 9 kb fragment in wild-type (ILK+) and 5 kb fragment in correctly targeted neo-positive knock-in (ILKki,neo) alleles. Cre recombinase-mediated excision of the neo-cassette results in a neo-negative knock-in (ILKki) allele, Ilktm10.1Ref. | ||||
| Copyright | This image is from Moik D, J Biol Chem 2013 Jun 28;288(26):18863-71 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:199658 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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