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| Caption | Generation of the Prkcitm1Ltg allele. Targeting of the Prkci (PKCi) locus in mice. Schematic representation of the targeting strategy is shown. Restriction maps indicating the relevant restriction sites of the wild-type (wt) and mutant locus are shown (B, BamHI; Bg, BglII; E, EcoRI; EV, EcoRV; H, HindIII; K, Kpnl). The black box represents the second exon of the Prkci gene which was used for all targeted modifications. The white boxes below the mutant alleles indicate the 5' HindIII-EcoRI RFLP probe (5' probe) and the 3' KpnI-EcoRI RFLP probe (3' probe) used for later southern blot analysis. A) conventional knockout - Prkci wt locus, the targeting construct (harboring an inserted neo cassette into the 2nd exon) and the mutant Prkci locus (PKCi Neo allele) after homologous recombination are shown. | ||||
| Copyright | This image is from Seidl S, PLoS One 2013;8(5):e62756, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:202124 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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