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| Caption | Gene targeting for the Pax1tm1.1Tlu (Pax1EGFP:Pax1+) construct. Genomic organization of Pax1 represented in a line diagram. Yellow boxes represent the exons (numbered accordingly); black solid lines, intronic sequences; white boxes, untranslated region (UTR); pink box, IRES; orange box, F2A peptide; red triangles, FRT sites; green boxes, EGFP; blue boxes, Neo cassette; purple solid lines, external Southern blotting probes; green solid lines, internal Southern blotting probes; blue arrows, primer pairs. E; EcoRI and St, Stu1. (a) Pax1tm1.1Tlu targeting construct depicting the insertion of the IRES-EGFP-FRT-PGK-gb2-Neo-FRT cassette (2.91 kb) in the 3' UTR of Pax1. The expected fragment size from Stu1 digestion for the mutant allele, as detected by hybridization Probes 1 and 2, is 7.81 kb (dotted line). Bottom: line drawing representing the allele after the FLPe mediated excision of Neo selection cassette in mice. | ||||
| Copyright | This image is from Sivakamasundari V, Genesis 2013 Jun;51(6):420-9, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:198603 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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