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| Caption | Generation of the Pkd1tm1Fqi (Pkd1NeoV) and Pkd1tm1.1Fqi (Pkd1V) alleles by gene targeting. (A) Structure of various Pkd1 alleles. (i) Targeting vector. Neo, FRT-flanked (red triangle) PGK-neomycin selection cassette; TK, thymidine kinase gene; S, SpeI. Exons are depicted by solid boxes. The T3041V mutation is indicated. (ii) Wild-type (WT) allele, with exon number shown. (iii) Pkd1tm1Fqi (Pkd1NeoV) allele. The expected size of the SspI or SpeI restriction fragments for WT and Pkd1tm1Fqi (Pkd1NeoV) alleles by using the 5' probe (within exon 15 outside of the vector) is shown. The expected size of the 3' long-range PCR product is also shown. (iv) Pkd1tm1.1Fqi (Pkd1V) allele. A single FRT site of 47 bp is retained at the site of Neo insertion. | ||||||
| Copyright | This image is from Yu S, Proc Natl Acad Sci U S A 2007 Nov 20;104(47):18688-93. Copyright 2007 National Academy of Sciences, U.S.A. J:127628 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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