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| Caption | Generation of the Mir122tm1.1Apts allele. (A) The strategy for generating mutant mice by homologous recombination. The BAC clone bMQ-418A13 (chr18:65269984-65437465) containing the entire pri-Mir122a locus was purchased from Geneservice. A genomic fragment of 13 kb encompassing 7.8 kb upstream and 5.1 kb downstream of pre-Mir122a was cloned to PL253 in bacterial strain EL350 by the recombineering-based method. The genomic fragment of Mir122a constructed in PL253 was used to replace the wild-type allele of Mir122a in 129 mouse embryonic stem cells (MESC). MESC clones containing the targeted allele were identified by Southern blot analysis. Several clones were isolated and transfected with a vector encoding the Cre recombinase to delete a fragment of 1,544 bp containing the entire pre-Mir122a. Clones with the Mir122a knockout allele (Mir122tm1.1Apts) were identified by Southern blot analysis and injected into the C57BL/6J blastocysts. Germline transmission of the Mir122tm1.1Apts allele was achieved by crossing the chimeric mice with normal C57BL/6 mice. | ||||
| Copyright | This image is from Tsai WC, J Clin Invest 2012 Aug 1;122(8):2884-97 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:190066 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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