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| Caption | Schematic for the generation of the Tgm3tm1Sjo allele. The locus of exons 4 to 7 of the TGM3 gene (A). The targeting construct was produced by insertion of the neomycin resistance cassette into exon 6. Southern blot analysis of cells after restriction digestion using NheI for the 5' probe and PstI with the 3' probe was used to identify correctly targeted ES cells. The disruption of exon 6 resulted in the wild-type 28.8 kb NheI fragment being reduced to 3.4 kb, and the 5.3 kb PstI fragment being reduced to 4.9 kb. Southern blot analysis of ES cell DNA after NheI digestion and hybridization with the 5' probe (B, left panel), and after PstI digestion and hybridization with the 3' probe (B, right panel). | ||||
| Copyright | This image is from John S, PLoS One 2012;7(4):e34252, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187109 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/30/2024 MGI 6.23 |
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