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| Caption | Targeted deletion of TRPV6 channel pore and C terminus. We used a Cre-loxP strategy to excise exons 13, 14, and 15, which encode the pore region, part of the fifth and the entire sixth transmembrane-spanning domains, and the cytosolic C terminus of TRPV6. a, the wild-type Trpv6+ allele and targeting construct. Translated exons (not in scale) are shown as filled boxes. Exons 13, 14, and 15 are flanked by loxP sites (filled triangles). An FRT site (gray triangles)-flanked PGK-neor cassette is located upstream of the second loxP site. B, BamHI; X, XhoI. Probes and sizes of genomic DNA fragments as expected by Southern blots are indicated. HSVtk, herpes simplex virus thymidin kinase. b, Cre-mediated conversion of the floxed allele to the Del(6Ephb6-Trpv6)2Mfre (Trpv6-/-) allele in mice. Cre-mediated excision of exons 13, 14, and 15 of the Trpv6 gene leads to deletion of exons 17 and 18 of the adjacent Ephb6 gene. | ||||
| Copyright | This image is from Weissgerber P, J Biol Chem 2012 May 25;287(22):17930-41 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:185629 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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