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| Caption | Generation of the 'negatively tagged' Clcn5tm5.1Tjj (clcn5Y672E*) allele. B, Targeting strategy. The targeting construct containing 12.5 kb of mouse genomic Clcn5 sequence was modified by inserting the Y672E mutation into exon 11 and by changing four base pairs in exon 12 that mutate two amino-acids of the extreme carboxy-terminus (indicated by an asterisk '*'). For positive selection, a FRT flanked neomycin-resistance cassette was inserted into intron 11 and for negative selection, a diphtheria toxin A (dta) cassette was added at the 5' end. In addition, an Acc651 (A) site (with a loxP site) was inserted into intron 9 and an EcoRV site was added in the 3' UTR for screening purposes (A and B). In vivo Flp-mediated deletion of the neomycin resistance cassette resulted in the Clcn5tm5.1Tjj (clcn5Y672E*) allele. | ||||
| Copyright | This image is from Rickheit G, J Biol Chem 2010 Jun 4;285(23):17595-603 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:164474 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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