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| Caption | The generation of conditional Spns2tm1.1Nmoc (Spns2f) and knock-out Spns2tm1.2Nmoc (Spns2-) alleles. (A) Schematic representation of the wild-type locus, the targeted allele, the floxed Spns2tm1.1Nmoc allele and the deleted Spns2tm1.2Nmoc allele. In the targeted allele, exon 2 of the Spns2 gene (yellow box) is flanked by two loxP sites (green arrowheads). Together with the first loxP site, a neomycin selection cassette, PKG-Neo-pA (gray box), flanked by two frt sites (orange arrowheads) has been inserted. Mice carrying the targeted Spns2 allele were crossed with transgenic mice expressing Flp recombinase under the control of cytomegalovirus early enhancer/chicken beta actin (CAG) promoter to remove the PKG-Neo-pA cassette, resulting in the floxed Spns2tm1.1Nmoc mice. To generate conventional Spns2tm1.2Nmoc knockout mice, the mice carrying a floxed allele were crossed with transgenic mice expressing the Cre recombinase under the control of cytomegalovirus (CMV) promoter. The floxed Spns2tm1.1Nmoc allele can be used to conditionally inactivate Spns2 in specific tissues by mating with mice that carry Cre recombinase driven by various promoters. | ||||||
| Copyright | This image is from Fukuhara S, J Clin Invest 2012 Apr 2;122(4):1416-26 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:184558 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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