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| Caption | (A) Targeting strategy. Top: wild-type (wt) allele containing the Airn promoter, below: targeting vector that deletes the TDRs (grey bar) lying in the 3' part of the CGI (green bar) with homology regions indicated. A floxed selection cassette (Pgk1 promoter driving a neomycin resistance gene (neo) stopped by a Pgk1 polyadenylation signal (pA); flanked on both sides by loxP sites) was inserted into the NheI site of Igl2r intron 3. Homologous recombination created the TDRdelta+cas allele. ES cells with a TDRdelata+cas allele were injected into blastocysts, mice carrying a targeted allele were identified and the selection cassette was removed by mating with a MORE-CRE expressing mouse to generate the Airntm2.1Dpb allele. Note the remaining loxP site in the mutant allele lies 1.3 kb upstream of the Airn promoter (black triangle). | ||||
| Copyright | This image is from Koerner MV, PLoS Genet 2012 Mar;8(3):e1002540, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:183397 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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