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| Caption | Generation of luciferase reporter Gt(ROSA)26Sortm9(CAG-luc)Npa (mR26CS-Luc) allele. (A) Flp-RMCE into the Gt(ROSA)26Sortm5Npa (modRosa26FRT) locus was performed, replacing the HygR in the Gt(ROSA)26Sortm5Npa ES cells with the FRT/FRT wt-flanked sequence from the Flp-RMCE targeting plasmid. The Flp-RMCE targeting plasmid contains the pCAG promoter followed by a LoxP-flanked (floxed) STOP cassette (STOP2), the luciferase cDNA and a NeoR cassette, flanked as a group by FRT3/FRT wt sites. Successfully targeted ES cells were used to generate Gt(ROSA)26Sortm9(CAG-luc)Npa mice. After crossing the mutant mice with Cre mice, ubiquitous or tissue-restricted luciferase reporter expression can be obtained to monitor reporter expression in living mice upon Luciferin injection. | ||||
| Copyright | This image is from Tchorz JS, PLoS One 2012;7(1):e30011, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:184312 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 01/20/2026 MGI 6.24 |
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