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| Caption | Generation of the reporter strain for strong EGFP expression, Gt(ROSA)26Sortm8(CAG-EGFP)Npa (mR26CS-EGFP). (A) Flipase-recombinase-mediated cassette exchange (Flp-RMCE) into the Gt(ROSA)26Sortm5Npa (modRosa26FRT) locus, replacing the HygR selection cassette in the Gt(ROSA)26Sortm5Npa ES cells with an FRT/FRT wt-flanked sequence in the Flp-RMCE targeting plasmid. The Flp-RMCE targeting plasmid contains the pCAG promoter followed by a LoxP-flanked (floxed) STOP cassette, the EGFP cDNA and a NeoR cassette, flanked as a group by FRT/FRT wt sites. Successfully targeted ES cells were used to generate Gt(ROSA)26Sortm8(CAG-EGFP)Npa mice. After crossing Gt(ROSA)26Sortm8(CAG-EGFP)Npa mice with several Cre mice, ubiquitous or tissue-restricted EGFP reporter expression could be obtained. | ||||
| Copyright | This image is from Tchorz JS, PLoS One 2012;7(1):e30011, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:184312 | ||||
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/21/2024 MGI 6.23 |
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