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| Caption | (A) Scheme depicting the generation of the Gt(ROSA)26Sortm5Npa (modRosa26FRT) allele in ES cells. A Stop sequence and a HygR selection cassette flanked by heterospecific FRT sites (FRT3 and FRTwt) were targeted to the Rosa26 locus between exons 1 and 2 by homologous recombination. After successful recombination, the Stop cassette is located downstream of the endogenous Rosa26 promoter. (B) Flp-RMCE was performed by introducing the Cdh5 (VeCad) or Acta2 (alphaSMA) promoter driving Cre recombinase into Gt(ROSA)26Sortm5Npa (modRosa26FRT) ES cells, which were then used to generate transgenic mice, Gt(ROSA)26Sortm7(Cdh5-cre)Npa (mR26-VeCad-Cre) or Gt(ROSA)26Sortm6(Acta2-cre)Npa (mR26-aSMA-Cre). The primers and probe for genotyping are indicated (1-3). | ||||||
| Copyright | This image is from Tchorz JS, PLoS One 2012;7(1):e30011, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:184312 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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