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| Caption | FIG. 1. Construction and characterization of VMAT2 knockout mice. (A) Representation of the wild type and predicted knockout genomic sequences, with prominent restriction endonuclease sites, neomycin-resistance sequences, and sequences recognized by the pVMAT2ko5' and pVMAT2ko3' hybridization probes as indicated. Construction of pBSneoV2KO targeting vector indicating prominent restriction endonuclease cleavage sites and sites for the neomycin resistance (Neo) and of the thymidine kinase (TK) sequences. (B) Southern blot analyses of hybridization of radiolabeled pVMAT2ko5' (Left) and pVMAT2ko3' (Right) hybridization probes with genomic DNA extracted from the tails of wild type (+/+), heterozygote (+/-), and homozygote (-/-) mice. Longer fragments result from homologous recombination replacing VMAT2 sequences including exons I-III with the neomycin-resistance cassette. (C) Scatchard analyses of saturation radioligand binding data using [3H]dihydrotetrabenazine and striatal membranes prepared from 6-week-old wild-type and heterozygous knockout mice and from whole-brain membranes prepared from postnatal day 1 wild type, heterozygote, and homozygote knockout animals (Inset). (D) Kaplan-Meier survival plots of wild type, heterozygous, and homozygous knockout mice. Approximately 15 mice of each genotype were followed for 2 months. (E) Weight gains of wild type (+/+) and heterozygote (+/-) knockout mice. | ||||
| Copyright | This image is from Takahashi N, Proc Natl Acad Sci U S A 1997 Sep 2;94(18):9938-43. Copyright 1997 National Academy of Sciences, U.S.A. J:42811 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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