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| Caption | Targeted deletion of exons 3 and 4 of the Usp2 gene. A, A diagram of a portion of the Usp2 gene is illustrated at the top with exon numbering based on Ensembl Gene structure of Usp2a (Usp2-201-ENSMUT00000034508). The targeting vector was constructed to introduce LoxP sites upstream and downstream of exons 3-4 as well upstream of the thymidine kinase (TK) neomycin (NEO) cassette. The structure of Usp2b (not shown) begins with an alternative exon 1 and exons 3-4 correspond to exons 2-3 in Usp2b (Usp2-202-ENSMUST00000065461). Transient transfection of targeted ES cells with a plasmid encoding Cre recombinase was expected to produce cells carrying a conventional knock-out allele, Usp2tm1.1Bjc, with a single LoxP site replacing exons 3 and 4 with insertion of a stop codon. Cre expression was also expected to produce a "floxed" allele, Usp2tm1Bjc (not illustrated), with LoxP sites in intron 2-3 and 4-5 with deletion of the TK-Neo cassette. Three Southern blot strategies for determining correct targeting involved restriction digests with enzymes (color coded) at the relative positions indicated in each diagram. Note that the BamHI site 59 of exon 3 and BstZ17I site 39 of exon 4 (each marked by an asterisk) were deleted from the wild-type gene by the targeting strategy. | ||||||
| Copyright | This image is from Scoma HD, PLoS One 2011;6(9):e25382, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:177916 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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