Phenotype Image Detail

Image

Caption

Morphology and composition of intestinal crypts in Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺ (TKO) mice. (A-D) Intestinal sections were stained with PAS/Alcian blue to label Paneth cells and goblet cells. Sections from wild-type (WT) (A) and TKO (B-D) mice are shown. The inset in (B) is magnified in (C). In (A), asterisks indicate CBC cells. In (D), asterisks indicate immature Paneth cells. Crypt borders are indicated with black hatched line. (Scale bar: 10 um.) (E and F) Intestinal sections were stained with the enterocyte-specific marker L-Fabp. Sections from WT (E) and TKO (F) mice are shown. Green, L-Fabp; blue, nuclei (DAPI). Hatched lines denote crypt margins. (Scale bar: 100 um.) (G) Musashi-1 and Dcamkl-1-positive cells are maintained in TKO mice. Green (Upper), Musashi-1; green (Lower), Dcamkl-1; red, BrdU; blue, DAPI. (Scale bars: 20 um.) Crypt borders are outlined. Arrows indicate cells staining negative for BrdU but positive for Musashi-1 or Dcamkl-1. (H) The number and location of 28 Mushashi-1-positive cells were determined in TKO mice. The cell at the base of the crypt was defined as position 1.

Copyright

This image is from Lee G, Proc Natl Acad Sci U S A 2009 Mar 24;106(12):4701-6. Copyright 2009 National Academy of Sciences, U.S.A. J:145768

Associated Alleles

Symbol	Name
Cdc25a^tm1.1Hpw	cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms
Cdc25a^tm1Hpw	cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms
Cdc25b^tm1Pjd	cell division cycle 25B; targeted mutation 1, Peter J Donovan
Cdc25c^tm1Hpw	cell division cycle 25C; targeted mutation 1, Helen Piwnica-Worms
Gt(ROSA)26Sor^tm1(cre/ERT)Brn	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^tm1(cre/ERT)Brn/Gt(ROSA)26Sor^+	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6