Gene Expression Image Detail

Reference

Figure

ID

J:229895 Sugiyama Y, et al., Mech Dev. 2016 Feb;139:10-7

2

MGI:6315238

Image

Caption

(A) Immunofluorescent staining of sections at E16.5. This shows lens epithelial (Epi) and fibre (Fb) cells in a region anterior to the equator of control [IFT88(fl/fl);MLR10(-)] and cKO [IFT88(fl/fl);MLR10(+)] lenses. Arrowhead indicates IFT88-stained cell (d',e',f'). Acetylated a-tubulin detected cilia, basal bodies and filamentous tubulin bundles in the elongating fibres, whereas the signal of beta-catenin was visible on the lateral membrane of the epithelial cells. IFT88 signals were detected as dots at the bases and the tips of cilia (bracket in a', b' and c' highlights a cilium which has two spots of signal at both ends. (B) Immunofluorescent staining of sections to show cellular alignment/orientation of lens fibres at E16.5. Cre-negative [IFT88(wt/fl);MLR10(-)] and Cre allele-positive [IFT88(wt/fl);MLR10(+)] controls and cKO [IFT88(fl/fl);MLR10(+)] lenses show a similar pattern of fibre cell alignment/orientation. Schematic drawings on the right side of each image outline overall fibre cell alignment. Sutures (S). Abbreviations for alleles: wt, wild-type; fl, floxed; d, deleted. For MLR10 Cre allele: +, positive; -, negative. Scale bars: A 20 um (upper panels) and 5 um (lower panels), B 200 um.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.mod.2016.01.003 Mech Dev 139: 10-7, Sugiyama Y; Shelley EJ; Yoder BK; Kozmik Z; May-Simera HL; Beales PL; Lovicu FJ; McAvoy JW, Non-essential role for cilia in coordinating precise alignment of lens fibres. Copyright 2016.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
Aa	MGI:6315340 (Ift88)
Aa'	MGI:6315340 (Ift88)
Ab	MGI:6315342 (Ctnnb1)
Ab'	MGI:6315342 (Ctnnb1)
Ac	MGI:6315342 (Ctnnb1) MGI:6315340 (Ift88)
Ac'	MGI:6315342 (Ctnnb1) MGI:6315340 (Ift88)
Ad	MGI:6315340 (Ift88)
Ad'	MGI:6315340 (Ift88)
Ae	MGI:6315342 (Ctnnb1)
Ae'	MGI:6315342 (Ctnnb1)
Af	MGI:6315342 (Ctnnb1) MGI:6315340 (Ift88)
Af'	MGI:6315342 (Ctnnb1) MGI:6315340 (Ift88)
Ba	MGI:6315342 (Ctnnb1)
Bb	MGI:6315342 (Ctnnb1)
Bc	MGI:6315342 (Ctnnb1)