Phenotype Image Detail

Image

Caption

KATNAL2 has essential roles in tail formation and centriole number regulation. Acetylated tubulin immunolabelling as a marker for sperm tails in wild-type (Katnal2^WT/WT, WT) (a) and Katnal2^m1Anu/Katnal2^m1Anu (Katnal2^Y86C/Y86C) (b) testis sections. Sperm tail content was markedly reduced in mutant versus WT. Scale bars in a-b = 10 um. In both WT (c) and mutant (d) spermatids, normal coupling of the centriole to the nuclear membrane was observed (black arrowheads). Docking of the centriole to the plasma membrane (white arrowhead) was frequently observed in WT spermatids (c), but was never seen in mutant spermatids (d). Supernumerary centrioles (red arrowhead) were frequently observed in the cytoplasm of mutant (d) but not WT (c) spermatids. Immunostaining of centrioles in isolated elongating spermatids of WT (e) and mutant (f) mice confirmed abnormal duplication of centrioles (red arrowhead) in mutant mice. In (e-f) red represents centrioles as labelled by centrin immunostaining and blue represents DNA as labelled by DAPI. Scale bars in c-f = 2 um.

Copyright

This image is from Dunleavy JEM, PLoS Genet 2017 Nov;13(11):e1007078, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:250115

Associated Alleles

Symbol	Name
Katnal2^m1Anu	katanin p60 subunit A-like 2; mutation 1, Australian National University

Associated Genotypes

Allelic Composition	Genetic Background
Katnal2^m1Anu/Katnal2^m1Anu	C57BL/6J-Katnal2^m1Anu