Phenotype Image Detail

Image

Caption

HSPB7 represses formation of abnormal actin bundles independent of Lmod2. (A) Immunofluorescence images of wild-type (WT), Lmod2^{tm1(KOMP)Vlcg}/Lmod2^{tm1(KOMP)Vlcg} (Lmod2 KO), Hspb7^tm1.2Chen/Hspb7^tm1.2Chen (HSPB7 KO), and Hspb7^tm1.2Chen/Hspb7^tm1.2Chen Lmod2^{tm1(KOMP)Vlcg}/Lmod2^{tm1(KOMP)Vlcg} (Lmod2/HSPB7 double KO; DKO) E10.5 heart sections stained with antibodies against HSPB7, Lmod2, and phalloidin (F actin.) (Scale bar: 100 mm.) (B) Representative immunofluorescence images (Left) and thin filament length measurements (Right) of control (Ctrl; including WT and Hspb7^tm1.2Chen heterozygous mice), Lmod2 KO, HSPB7 KO, and Lmod2/HSPB7 DKO E10.5 embryos. Two embryos, 50-78 sarcomeres measured for each group. Error bars indicate mean SEM. Control, 0.849 0.0069 mm; Lmod2 KO, 0.77 0.0066 mm; HSPB7 KO, 0.885 0.0074 mm; DKO, 0.802 0.006 mm. Statistical significance was determined with one-way ANOVA. H, actin free H zone; Z, Z lines. **P < 0.01 (Ctrl vs. HSPB7 KO, P = 0.0033; Lmod2 KO vs. DKO, P = 0.0021); ****P < 0.0001. (Scale bar: 1 um.) (C) Representative immunofluorescence images of WT, Lmod2 KO, HSPB7 KO, and Lmod2/HSPB7 DKO E10.5 heart cryosections stained with antibodies against Tmod1, alpha-actinin (Actn2), and phalloidin (F actin). Abnormal actin bundles (AABs) are indicated by yellow arrowheads. (Scale bar: 10 mm.)

Copyright

This image is from Wu T, Proc Natl Acad Sci U S A 2017 Nov 7;114(45):11956-11961. Copyright 2017 National Academy of Sciences, U.S.A. J:256653

Associated Alleles

Symbol	Name
Hspb7^tm1.2Chen	heat shock protein family, member 7 (cardiovascular); targeted mutation 1.2, Ju Chen
Lmod2^tm1(KOMP)Vlcg	leiomodin 2 (cardiac); targeted mutation 1, Velocigene
Lmod2^tm1(KOMP)Vlcg	leiomodin 2 (cardiac); targeted mutation 1, Velocigene

Associated Genotypes

Allelic Composition	Genetic Background
Hspb7^tm1.2Chen/Hspb7^tm1.2Chen Lmod2^tm1(KOMP)Vlcg/Lmod2^tm1(KOMP)Vlcg	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * C57BL/6NTac * CBA