Gene Expression Image Detail
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Reference
Figure
ID
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J:264612
Morgani SM, et al., Dev Biol. 2018 Sep 1;441(1):104-126
9
MGI:6285970
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| Caption | A,B,D,H. Representative wholemount images of somite stage Spry4H2B-Venus/+ embryos, showing either Venus fluorescence alone or merged with the brightfield image. A. Lateral view of E8.5 embryo. B. Lateral view of E8.75 embryo. D. Lateral view of E10.5 embryo. Limb buds were removed and imaged at higher magnification (lower panels). H. Lateral and dorsal view of E12.5 embryo. Limbs and tail were removed and imaged at higher magnification (lower panels). Ss,somite stage; tb, tail bud; pa I/II, pharyngeal arch one and two; fl, forelimb; hl, hindlimb; fnp, frontonasal process; mx, maxillary arch; ma, mandibular arch; np, nasal placode; is,isthmic constriction; aer, apical ectodermal ridge; se, surface ectoderm; A, anterior; P, posterior; D, dorsal; V, ventral; Pr, proximal; Ds, distal; L, left; R, right. C. Wholemount in situ hybridization labeling in an E10.5 embryo. E. Confocal image of a cryosectioned forelimb bud from an E10.5 embryo showing Spry4H2B-Venus alone and as a merge with the nuclei stain (Hoechst). Inset shows high magnification region of AER. AER, apical ectodermal ridge. Scale bars, 50 um. F. Confocal image of an E10.5 cryosectioned limb bud depicting the arrangement of example measurement lines used for the quantification in panel G. Four lines were made along the proximal to distal axis of the limb bud (1-4) and four across the anterior-posterior axis (A-D). G. Quantification of signal gradient in E10.5 forelimb limb buds (n = 5 limb buds, 5 cryosections per limb bud). Each dot represents the average fluorescence intensity per limb bud at a particular point along the line. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Copyright | Reprinted with permission from Elsevier from 10.1016/j.ydbio.2018.06.017 Dev Biol 441: 104-126, Morgani SM; Saiz N; Garg V; Raina D; Simon CS; Kang M; Arias AM; Nichols J; Schroter C; Hadjantonakis AK, A Sprouty4 reporter to monitor FGF/ERK signaling activity in ESCs and mice. Copyright 2018 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Associated Assays |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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