Phenotype Image Detail

Image

Caption

The sperm axoneme from Cfap43^em1Pfr/Cfap43^em1Pfr (Cfap43-/-) males is fully disorganized. a) Central pair is absent in sperm from mutant males. Staining of wild-type (WT) sperm with an anti-Spef2, a marker of the projection 1b of singlet C1 (a, green) and anti-acetylated tubulin (b, red) antibodies. c) Overlay of Spef2 and tubulin staining. Sperms were counterstained with Hoechst (blue). d-f) Similar experiments on sperm from mutant males. Scale bars = 5 um. g-l) Head of radial spoke are absent in sperm from mutant males. Staining of WT sperm with an anti-Rsph4a, another protein of the RS head (g, green) and anti-tubulin (h, red) antibodies. i) Overlay of Rsph4a and tubulin staining. Sperm were counterstained with Hoechst (blue). j-l) Similar experiments on sperm from mutant males. Scale bars = 5 um. m, n) Transversal section of a sperm from WT (m) and mutant (n) males observed by EM in the midpiece region. Note the specific arrangement of the outer dense fibers (ODFs) around the axoneme in WT sperm and the complete disorganization of the doublet of microtubules (DMTs) and the absence of the central pair in mutant sperm. Scale bars = 240 nm. Number of cross-section for WT and mutant were 21 and 30, respectively. All cross-section were defective for mutants. DMTs, doublet of microtubules; ODF, outer dense fiber; Mt, mitochondria.

Copyright

This image is from Coutton C, Nat Commun 2018 Feb 15;9(1):686, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:257803

Associated Alleles

Symbol	Name
Cfap43^em1Pfr	cilia and flagella associated protein 43; endonuclease-mediated mutation 1, Pierre F Ray

Associated Genotypes

Allelic Composition	Genetic Background
Cfap43^em1Pfr/Cfap43^em1Pfr	involves: C57BL/6J