Phenotype Image Detail

Image

Caption

Spermiogenesis defect in Tdrd6^tm1Chum/Tdrd6^tm1Chum males. (R) Gross appearance of Tdrd6^tm1Chum/Tdrd6⁺ and Tdrd6^tm1Chum/Tdrd6^tm1Chum testes at 2 months. (S-U) H&E-stained sections of heterozygous (S) and homozygous (T and U) testes at 2 months. The arrow indicates mature spermatozoa. In homozygous testes (T and U), elongating spermatids were observed, but were reduced in number and had aberrant nuclear morphologies (arrowheads). sc, spermatocytes, rs, round spermatids, es, elongating spermatids. (V and W) H&E-stained sections of heterozygous (V) and homozygous (W) epididymides at 2 months. The arrow indicates mature spermatozoa. (X and Y) TUNEL staining (red) of apoptotic cells in heterozygous (X) and homozygous (Y) testis sections counterstained with a Hoechst dye (blue). (AA and AB) Acrosome staining [peanut agglutinin (PNA), red] of heterozygous (AA) and homozygous (AB) spermatids counterstained with a Hoechst dye (blue). Mutant spermatids (AB) had elongating nuclei with acrosomes spreading over (steps 13-14 spermatids). (Scale bars: F-I, P, Q, and S-Y, 25 um; R, 1 mm; AA and AB, 10 um.

Copyright

This image is from Tanaka T, Proc Natl Acad Sci U S A 2011 Jun 28;108(26):10579-84. Copyright 2011 National Academy of Sciences, U.S.A. J:173550

Associated Alleles

Symbol	Name
Tdrd6^tm1Chum	tudor domain containing 6; targeted mutation 1, Shinichiro Chuma

Associated Genotypes

Allelic Composition	Genetic Background
Tdrd6^tm1Chum/Tdrd6^tm1Chum	involves: 129S4/SvJae * C57BL/6