Phenotype Image Detail

Image

Caption

Tip cell abnormalities in Nrp2^tm1Ddg/Nrp2⁺ Flt4^tm1Ali/Flt4⁺ (nrp2+/- vegfr3+/-) mice are similar to those obtained after anti-Nrp2B treatment. (A and B) X-gal staining of P5 hearts from mice of the indicated genotypes. Note regular branching of lymphatics in the Flt4^tm1Ali/Flt4⁺ (vegfr3+/-) heart (A) but enlarged, poorly branched vessels devoid of sprouting tips in the double-heterozygote heart (B). (C-F) Lymphatic sprouting defects in the small intestine at P4. LYVE-1-stained sections show presence of lymphatics in almost all villi of wild-type (WT) mice (C) but virtually no sprouts in most sections of the double-heterozygous mice (D). Only some sections show a portion of the villi-containing lymphatics (E), which are of normal length compared with WT (C). (F) Quantification of the percentage of villi containing lymphatics. *, P < 0.01. Error bars indicate SEM. Bars: (A and B) 210 um; (C-E) 200 um.

Copyright

This image is from Xu Y, J Cell Biol 2010 Jan 11;188(1):115-30, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:156869

Associated Alleles

Symbol	Name
Flt4^tm1Ali	FMS-like tyrosine kinase 4; targeted mutation 1, Kari Alitalo
Nrp2^tm1Ddg	neuropilin 2; targeted mutation 1, David D Ginty

Associated Genotypes

Allelic Composition	Genetic Background
Flt4^tm1Ali/Flt4^+ Nrp2^tm1Ddg/Nrp2^+	involves: C57BL/6