Phenotype Image Detail

Image

Caption

Embryonic lethality in double Nhej1^tm1Fwa (Xlf-) and Paxx^em1Spj (Paxx-) homozygotes. Embryos from timed mating were isolated at the indicated stage of embryonic development, imaged for morphological assessment, and genotyped by PCR from the yolk sacs or tail snips. (A) Representative images of Nhej1^tm1Fwa/Nhej1^tm1Fwa and Paxx^em1Spj/Paxx^em1Spj Nhej1^tm1Fwa/Nhej1^tm1Fwa E10.5 littermate embryos. (D) Representative images of mutant and double mutant E14.5 littermate embryos. (G) Representative images of Nhej1^tm1Fwa/Nhej1^tm1Fwa (left) and double mutant E18.5 littermate embryos. (I) Representative images of thymi and spleens of E18.5 live embryos.

Copyright

This image is from Balmus G, Genes Dev 2016 Oct 1;30(19):2152-2157, and is displayed with the permission of Cold Spring Harbor Laboratory Press, who owns the Copyright. J:236776

Associated Alleles

Symbol	Name
Nhej1^tm1Fwa	non-homologous end joining factor 1; targeted mutation 1, Frederick W Alt
Paxx^em1Spj	non-homologous end joining factor; endonuclease-mediated mutation 1, Stephen P Jackson

Associated Genotypes

Allelic Composition	Genetic Background
Paxx^em1Spj/Paxx^em1Spj Nhej1^tm1Fwa/Nhej1^tm1Fwa	involves: 129S6/SvEvTac * C57BL/6NTac