Phenotype Image Detail

Image

Caption

Defects in cerebellar development in Dnm1l^tm1.1Hise/Dnm1l^tm1.2Hise En1^tm2(cre)Wrst/En1⁺ and Dnm1l^tm1.2Hise/Dnm1l^tm1.2Hise En1^tm2(cre)Wrst/En1⁺ (both referred to as En1-Drp1KO) mice. (A) Immunofluorescence of cerebellum (Cb) at P1 using anti-Dnm1l antibodies. (B) H&E stains of sagittal sections of cerebella. (C) Area of cerebella in sagittal sections around the median line was determined (n = 3). (D and E) Sagittal cerebellar sections were immunostained using antibodies to Ki67 (D) or activated caspase-3 (E). (F) EM of Pukinje cells (PCs) at P0. Arrows indicate mitochondria. Sagittal sections of cerebella are shown. (G) Quantitation of mitochondrial shape and size in PCs. (H) EM of granule cells (GC) in the external granular layer (EGL). Sagittal sections of cerebella are shown. (I) Immunofluorescence of sagittal cerebellar sections around the median line using anti-Car8 antibodies. PCL, PC layer. (D, F, and H) Boxed areas are enlarged in the corresponding panels below. *, P < 0.05. Error bars indicate mean +/- SEM.

Copyright

This image is from Wakabayashi J, J Cell Biol 2009 Sep 21;186(6):805-16, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:153835

Associated Alleles

Symbol	Name
Dnm1l^tm1.1Hise	dynamin 1-like; targeted mutation 1.1, Hiromi Sesaki
Dnm1l^tm1.2Hise	dynamin 1-like; targeted mutation 1.2, Hiromi Sesaki
En1^tm2(cre)Wrst	engrailed 1; targeted mutation 2, Wolfgang Wurst

Associated Genotypes

Allelic Composition	Genetic Background
Dnm1l^tm1.1Hise/Dnm1l^tm1.1Hise En1^tm2(cre)Wrst/En1^+	involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Dnm1l^tm1.1Hise/Dnm1l^tm1.2Hise En1^tm2(cre)Wrst/En1^+	involves: 129 * C57BL/6 * FVB/N * SJL